Unit powers. At that point halfway up the maximum transmittance value, the total width (nm) of the transmitted band is. Wavelength range: 340-850 nm. The VersaMax and SpectraMax 190 read 96-well microplates. Absorbance measurements were recorded using a SpectraMax 340 (Molecular Devices) at 490 nm on the indicated days (Cell Proliferation Kit I; Roche). Permeability of the monolayer was verified by adding 1% Evans Blue dye (Thermo Fisher Scientific, Inc. Temperature Range: Ambient + 4 °C up to 45 °C. Like CD3, the CD95 death receptor requires binding of immobilized or multimeric antibodies to be activated effectively. 5. Microplate Readers. TheSpectraMax®190MicroplateSpectrophotometer,SpectraMax®340PC384Microplate Spectrophotometer,andVersaMax™MicroplateSpectrophotometerproviderapidandsensitive. , Sunnyvale, CA). This is a used Molecular Devices SpectraMax 340PC Microplate Spectrophotometer. Labsystems Luminoskan RT Microplate Reader. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Nitric Oxide Assay. 314 low lamp level warning. Stat Fax 4700 Microstrip Reader. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. 303 unable to cal dark current. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. 2 g/L solution; dilute 1:10 in HBSS. Figure 1. below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Briefly, hPDLSCs were cultured in osteogenic/odontogenic induction medium for 14. The cells were pretreated, serum starved for 24 h, and then treated with NK-cdM priora SpectraMAX 340VR microplate reader (Molecular Devices, Sunnyvale, CA, USA). or microplate reader (e. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 3 1. using SpectraMax i3 (Molecular devices, San Jose, USA). Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. 0, MSD) or Spectramax 340 (Molecular Devices). 2. ” SpectraMax® Quant™ dsDNA Assay Kits The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. Subsequently, the mixture was heated at 100 °C for 10. The GH Pro 340 Supplemental Greenhouse LED grow lighting is designed with greenhouse operations in mind. On LabX buyers can find a variety of SpectraMAX models for sale: 190, 384, 340 and the SpectraMAX M series. 3. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. LDH release was measured with a commercial kit following the manufacturer’s instructions (LDH Cytotoxicity Detection Kit, TaKaRa). Absorbance was measured at 450 nm by using a SpectraMax 340 microplate spectrophotometer (Molecular Device). Subsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. Plates were read at a wavelength of 405 nm with a reference wavelength of 620 nm (Spectramax 340 PC). One plate carrier adapter is provided with the instrument. application or budget. StakMax. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. SpectraMax Multi-Mode Microplate Readers. Product Keyword. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. 5. Introduction The SpectraMax® 340PC384 Reader from Molecular Devices provides everything needed to measure absorbance in the visible range, including temperature. SpectraMax® 340PC384 microplate reader provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro data analysis software. Thin layer chromatography (TLC) was. Acceptor: 665/10 nm. NHDFs were seeded in microtiter plates (96 wells) at a density of 1x105 cells per well. The initial rate of hydrolysis was determined using the early linear region of. using SpectraMax i3 (Molecular devices, San Jose, USA). The Spectra Catalina 340z produces 14 gallons (53 liters) of water per hour, making it the perfect watermaker for the midsize yacht with two to four people onboard. gp70 blocking. ZERO BIAS - scores, article reviews, protocol conditions and moreAbsorbance was measured at 450 nm with ELISA Reader (SpectraMax 340, Molecular Device Co. plates can be used in the SPECTRAmax PLUS 384. To screen for antigen specific IgG, 50 μL/well of biotinylated antigen at a concentration of 20-50 ng/mL was added and incubated for one hour at RT. Revvity. Read up to 6 wavelengths in endpoint or kinetic mode for 96- and 384-well plates, and perform spectral scans to determine. Awareness Technology, Inc. The increase in fluorescence was determined for 20 min by excitation at 520 nm and emission at 545 nm in a. The activity of generated thrombin was measured by the amidolysis of its substrate S-2238 and theThe purple formazan crystals formed were dissolved by adding 100 μL of DMSO to each well and absorbance was read at 570 nm in a spectrophotometer [SpectraMax 340]. Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. On 1, 7, 14, and 21 days, the cells were washed with DPBS twice and incubated with CCK-8 solution for 2 h in the dark at 37 °C. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1. The concentration of TNFα in the experimental samples was calculated from a mouse TNFα standard curve of 15–2000 pg/ml. , Sunnyvale, CA) after incubation of solution mixture at 37 °C for 2 h 30 min in the dark. Briefly, 50 µl-samples were incubated with equal volumes of Griess reagent (1% sulphanilamide, 0. 0. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Molecular Devices SpectraMax M5e Microplate Reader. The SpectraMax 340PC384 Microplate reader supports various absorbance assay categories, such as protein quantitation, endotoxin detection, microbial growth, ELISAs. Bacterial growth was measured after every 45 min by recording the absorbance at 595 nm using a SpectraMax 340 spectrophotometer (Molecular Devices, Sunnyvale, CA, USA). The SpectraMax 384 Plus lets you get absorbance measurements from 190 to 1000 nm quickly for samples in test tubes, cuvettes, and 96- or 384-well microplates. SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. Male 8 pin mini Din to Female 9D. Also for: Spectramax 190, Spectramax 340pc384, Versamax. Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536 The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. SpectraMax 250 With Computer SoftwareSpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. Enzyme activities at lower concentrations were assayed by using a LS50B fluorometric. As a common indicator of equine T cell activation, cytokine induction. Create custom fluorescence assays without having to purchase expensive filter sets. 0200-6117. Subsequently, the mixture was heated at 100 °C for 10. One plate carrier adapter is provided with the instrument. Incubation with TMB substrate was done for 18 min. Plasma glucose levels were measured using FreeStyle Lite (Abbott Diabetes Care Ltd. SpectraMax 340PC384 reads 96-well plates and 384-well plates and strip-well plates. Acceptor: 665nm. 8. The mixture was centrifuged for 10 min at 10,000 rpm, and BSA concentration in the supernatant was determined using bicinchoninic acid (BCA) assay. 04. The ability of PTK787/ZK 222584 to inhibit angiogenesis was tested in an in vitro model of capillary sprout formation (64) . Tunable for filterless flexibility. 5, 2 mM pNPP, and recombinant enzymes, PTP1B activities were continuously monitored on a SpectraMax 340 Microplate Reader (SpectraMax, Silicon Valley, CA, USA) at 405 nm for 2 min at 30°C. Here, we show how the SpectraMax® i3x and SpectraMax® iD5 Multi-Mode Microplate Readers are used to perform robust, no-wash saturation binding assays using HTRF. The cell concentration was measured by a spectrometer (SpectraMax 340, Molecular Devices, USA) with an optical density at 600 nm (OD 600). 85%. SpectraMAX 340 microtiter plate reader. reader (Molecular Devi ces). 81. TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytesacross. 001/min at 425 nm. Absorbance plate reader, temperature control, robotics-compatible, 384 well plates, 340-850 nm. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. Media in plates were withdrawn by pipetting and 200 μl of DMSO was added to each well to solubilize the formazan crystals. Scanning was done at 600 nm, using a Molecular Devices SpectraMax 340 plate reader. Up for your consideration is a Molecular Devices SpectraMax 340 Microplate Spectrophotometer Model# 340 Condition is for parts or repair. DNA extraction from blood meal ELISA homogenate. Samples with OD values of less than 0. SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. Labsystems Luminoskan RT Microplate Reader. , Sunnyvale, CA, USA) at 595 nm. 315 can't find zero order. Emission. #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. 190–850 nm <± 1. 4) was followed according to Vanacker et al. 2 - 3. Adhesion molecule expression To determine whether baicalein could modify the oxLDL-induced adhesion molecule expression, HUVECs were grown to confluence, pretreated with baicalein for 2 hrs and stimulated with oxLDL (150 μg/ml) for. PathCheck SensorThe optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. Subsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode Opening the Drawer Manually on page The SpectraMax ABS can accommodate standard 96-well plates. ELISAs were read with a Spectramax 340 from Molecular Devices (Sunnyvale, CA, USA). Although not statistically significant, pain was reduced by 2 NRS steps or more during supplemental treatment with naloxone in 36% of subjects when. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. HTRF-compatible SpectraMax Multi-Mode Readers. The absorbance values were measured at 450 nm using a SpectraMax® 340 plate reader (Molecular Devices, Sunnyvale, CA, USA). The drawer will open and it may close, if is doesn't, close it using the DRAWER button found on the Spectramax control panel (pictured in purple on. The Molecular Devices SpectraMax 340PC384 Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument. Microscopy. Values were determined according to a standard curve (log/log curve-fit). 1x10 5 transduced, GFP + SK-NEP-1. The estimation of monodehydroascorbate reductase (MDHAR) (EC 1. With SOFTmax PRO, the contents of the wells in a microplate can be mixed the industry-leading microplate reader software for the SpectraMax Plus 384 system, allows complete customization of data collection and analysis. The values of IC 50 represent concentrations of compounds toA microplate reader (SpectraMax M5; Molecular Devices, Sunnyvale, CA) was used to measure the absorbance at 340 nm for the collected BPW (6) Stabilization protects islet integrity during respirometry in the Oroboros Oxygraph-2K analyzer Islets December 31, 2022 Justin J. The SPECTRAmax 340PC can mea-The extracted stain was then transferred to a 96-well plate, and the absorbance at 562 nm was measured using a SpectraMax 340 plate reader/spectrophotometer(Molecular Devices Corp. This is a used Molecular Devices SpectraMax 340PC Microplate Spectrophotometer. Detection limits. The Gemini EM Reader with dual monochromators allows researchers to try new and novel dyes without having toThe absorbance was determined to be 450 nm using an ELISA reader (SpectraMax® 340, Molecular Device Co. 7. Changes in OD 405 nm were followed on the SpectraMAX 340 plate reader. The reaction mixture was incubated for 5 min. Availablility: By Availability Molecular Devices SpectraMAX 340 Microplate Reader. Triton X-100 (see Note 2). The release of p-nitrophenol ( 400) 17000 M-1 cm-1) was monitored at 25 °C for the enzymatic hydrolysis of com-pounds 1-8. Although it has been proven to be effective in various neurologic disorders, the mechanism of the treatment of traumatic brain injury (TBI) is not fully understood. , Sunnyvale, CA) at 490 nm. Donor: 616/10 nm. P. . Application note showing how SpectraMax® i3x and SpectraMax®iD5 Multi-Mode Microplate Readers are used to performGq-coupled receptor assays using HTRF technology. Irene Tieleman, Ido PenandSimon VerhulstSubsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. ELISA plates were read with a Spectramax 340 from Molecular Devices (Sunnyvale, CA, USA). The absorbances were being measured at 515 nm. The plate was covered and incubated for one hour at room temperature. The screening was initiated by adding 10 μL p-nitrophenyl phosphate (pNPP), and the catalysis of pNPP (1 mM) was continuously monitored on SpectraMax 340 microplate reader (Molecular Devices, Sunnyvale, CA, USA) at 405 nm for 20 min at 37 °C. 05% at 340 nm Photometric stabilization Instantaneous Photometric drift None—continuous referencing of monochromatic output Calibration Automatic before first kinetic read and. Lactate dehydrogenase (LDH) release assay Cytotoxicity was determined by measuring the release of LDH. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. 00 USD. With optimized reagents and the industry leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax to perfectly match your needs. SpectraMax190,340PC384,Plus384,VersaMaxMicroplateSpectrophotometersUserGuide 4 0112-0126D SafetyInformation. Immunofluorescence and Aggregation Analysis. 8 pin mini DIN (Spectramax 340) to 9D (PC) serial data transfer cable. About The SpectraMax GEMINI EM is controlled by an external computer running SoftMax ® Pro software which provides integrated instrument control, data display, and statistical data analysis. Liquid handling for random screening was carried out with a Biomek FX from Beckman Coulter (Fullerton, CA, USA) and HYTRA. For cell permeabilization, 1 ml of Z buffer (60 mM Na 2 HPO 4 z 7H 2 O, 40 mM NaH 2 PO 4 z H 2 O, 10 mM KCl. Percent Transmission Absorbance Competitor 0. The concentration of NO in the cells was determined by measuring the amount of nitrite, a relatively stable oxidation product of. Assay setup for a 384-well low-volume plate. Growth was monitored at 30°C every 6 min for 8 to 12 h at 660 nm using a multiscan photometer (spectraMax 340; Molecular Devices). , Fremont, CA, USA). SpectraMax Multi-Mode Microplate Readers. The ratio of red to green fluorescence intensity was calculated. Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. Plate reader (SpectraMax 340 PC plate reader or comparable plate reader). They are for reference only. The contents of the. The activity was measured in a SpectraMAX 340 plate reader at 37°C and 410 nmol/l for 120 sec. The SpectraTest validation package provides a NIST-traceable solution for validating optical performance of the SpectraMax Plus 384 reader and does it automatically. SpectraMax Multi-Mode Microplate Readers. Or scan up to 384 wells at any time to determine the best assay conditions. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Statistical analysis The significant differences between the different strains in the adherence assays were analyzed using the Student’s t -test. A microplate reader asThe absorbance was measured at 425 nm and read every 10 min using a Spectramax 340 plate reader (Molecular Devices ®, USA). Cell Culture AO391 (derived from human aorta) and CD314 (derived from human carotid artery) cell lines were a generous gift from Wendy Bauer (Tufts Molecular Cardiology Research Institute) and were grown in DMEM (CellGro) supplemented with 10% FBS. Spectral Range. 003 OD Stray light ≤ 0. 8 pin mini DIN (Spectramax 340) to 9D (PC) serial data transfer cable. Exhibit with us {{>currencyLabel}} Back. The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance. Anti-proteolytic activity. AoSMCs were seeded into 6-well culture plates. TheSpectraMax®Plus384addstheabilitytoreadcuvettes. The activity of glutathione peroxidase (GPX) was quantified spectrophotometrically at 340 nm, following the method of Takeda et al. Male 8 pin mini Din to Female 9D. Lactate dehydrogenase (LDH) release assay Cytotoxicity was determined by measuring the release of LDH. The SpectraMax i3x reader provides the flexibility to add novel detection capabilities without the need for service engineers or costly downtime—it grows with you as your research areas expand. 340/80 nm excitation with 616/10 nm emission range Optimized for use with ScanLater Western Blot Detection cartridge for the SpectraMax® i3, i3x, and Paradigm® Multi-Mode Readers Optimized for use with the enhanced TRF detection module for the SpectraMax iD5 Multi-Mode Microplate ReaderThe optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. 10. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1. MMP‑1 inhibition assay. 340 nm. Absorbance was read at 405 nm on a microplate reader (SPECTRAmax 340 PC). SpectraMax Multi-Mode Microplate Readers. #N207) LAL Reagent Water (Lonza cat. SoftMax Pro. Immunofluorescence and Aggregation Analysis. The plates were read using an ELISA microplate reader (Molecular Devices SPECTRAMAX 340 PC 384, Sunnyvale, California, USA) at 450 nm. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. After washing, the dye was eluted with 3% HCl and the absorbance measured at 665 nm with a SpectraMax 340 spectrophotometer (Molecular Devices, Sunnyvale, CA). The adapter is. Description Control Panel Cuvette Chamber Back Panel Microplate Drawer The Control Panel TEMP on/off TEMP Figure 1. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. 312 gain calibration failed. 6. Created Date: 20051213090753Z measured using a SpectraMax 340 multiwell plate. Introduction. Awareness Technology, Inc. Acceptor filter: 665/10 nm. Molecular Devices. Warranty. Absorbance was measured at 490/650 nm by using a SpectraMax 340 microplate spectrophotometer® (Molecular Devices, Sunnyvale, CA, U. All absorbance measurements were taken on a SpectraMax 340 PC reader equipped with SoFTMax Pro software. Details. Fig. TDP-43 stress granular cells were treated with 70% ice-cold. The absorbance was measured at 550 nm with a Spectramax 340 spectrophotometer (Molecular Devices, Sunnyvale, CA). Total Cell Count in BALF Cells collected from the lavage fluid were suspended in 1 mL of F-12 media supplemented with 10% FBS. 10× trypsin 0. Created Date: 20051213090753Zmeasured using a SpectraMax 340 multiwell plate. It measures the optical density (OD) of samples in both 96- and 384-well microplates at a selected wavelength for a single point in time (endpoint), over a specified period of time. Lightgreenglass,2 mmthick(340 nm) 9:Yellowglass,3 mmthick(405 nm) 10:Darkredglass,3 mmthick(650 nm)Bob - 06 Apr. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deepThe SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. . 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. 340 to 740 nm (linear variable dichroic) 220 to 1000 nm for abs (spectrometer) Plate Formats: 6 to 384-well plates, LVis Plate with sixteen 2 µL microspots. To. 5. Service Contracts, Preventative Maintenance and Performance. 6, 2 mmol/l DTT, 10 mmol/l MnCl 2 and 10 mmol/l pNPP; the PP2Cα enzyme concentration was 400nmol/l. To measure the cell biomass (g/L),. These isotypes cause antigen cross-linking by multimeric binding and self. Samples were analyzed in triplicates. Each experiment was performed in duplicate and repeated at. , Sunnyvale, Calif. Cell viability wasDescription Microplates The SpectraMax Multi-Mode Microplate Reader can accommodate SBS- standard 6-well to 384-well microplates and strip wells. A 0. 7 macrophages were obtained from ATCC (Manassas, VA). Awareness Technology Inc. TGFα release was calculated as described in the Results. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Absorbance values were measured using a plate reader (Molecular Devices; SpectraMax 340) at λ = 405 nm. The extreme flexibility and high sensitivity of the SpectraMax GEMINI EM make it appropriate for applications within the fields of bio-A microplate reader that can detect two or more applications is considered a multi-mode plate reader. 6, 2 mmol/l DTT, 10 mmol/l MnCl 2 and 10 mmol/l pNPP; the PP2Cα enzyme concentration was 400nmol/l. Molecular Devices SpectraMAX 340 Microplate Reader. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. After adding the stop solution, the absorbance at 450 nm with a wavelength correction at 540 nm was measured with a SpectraMax 340 plate reader (Molecular Devices, San. California Lightworks uses only the highest quality LEDs from Osram. The optical density at 405 nm (OD 405) of the plates was measured with a microplate reader (SpectraMax 340 PC384, Molecular Devices) before and after a 1-h incubation at room temperature. Fios Router Setup Manual, Losi Xx4 We Manual, Statics Hibbeler Solutions Manual 12th Edition, Nokia Hs 23 Manual, Spectramax 340 Manual, Nolan N102 User Manual, Ship Security Officer Manual yunqian 4. 2. MCE has not independently confirmed the accuracy of these methods. Please refer to photos for further. All strains were tested three times and the results averaged. SpectraMax Multi-Mode Microplate Readers. 2. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. Products : SpectraMax Plus 384 reader,. 5% phosphoric acid) in microplates at room temperature for 10 min. View the Molecular Devices website. 0200-0729. GE Healthcare spectramax 340 pc plate reader Spectramax 340 Pc Plate Reader, supplied by GE Healthcare, used in various techniques. The sensitivities of the hormones are Prolactin: 0. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. IC 50 values were determined by mathematical curve-fitting (SoftMaxPro Molecular Devices) and were defined as the drug concentration leading to 50% inhibition of net cell mass. SpectraMax® Microplate Readers and SoftMax® Pro Software. Wallac 1450 Microbeta TRILUX Liquid Scintillation & Luminescence Counter $1,000. Call 1-800-745-2710. 5 mL of 1 M acetate buffer (pH 4. Versteegh, Yoran H. Each experiment was performed in duplicate. The absorbance at 540 nm was determined in a spectrophotometer (SPECTRAmax 340, Molecular Devices). SpectraMax 340 (Serial number starting with M) SpectraMax 340PC (Serial number starting with L) SpectraMax Plus (Serial number starting with P) Tmax Also refer to the Obsolete Microplate Reader Models article for additional information. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. Absorbance was then measured at 540 nm (isosbestic point of cytochrome c) and 550 nm (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). 0. ir Neuroprotection of a sesamin derivative, 1, 2-bis [(3-methoxy- phenyl) methyl] ethane-1, 2-dicaroxylic acid (MMEDA) againstEach SpectraMax® 340 PC 384 Microplate Reader has an optical system built around a monochromator. Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. PerkinElmer offers a variety of Microplates For Absorbance and Colorimetric Assays. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Molecular Devices SpectraMax M5e Microplate Reader $26,500. 0 3. Cell permeabilization. 340 nm / 80 nm. Samples were plated in duplicate (r=0. 2. Below is the list of Molecular Devices microplate readers and liquid handling instruments that are of out-of-production and are now considered obsolete. #W50-640) SpectraMax i3 or i3x Multi-Mode Microplate Reader (Molecular Devices; other SpectraMax microplate readers with fluorescence detection are also suitable) SoftMax Pro Software (Molecular Devices). Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). Not all manufacturers' microplates are the same with regard to design, materials, or76358-624. Amino Acid Analysis. 5. Wells were washed three times. Filterless readers provide consistent results with steady temperature regulation. TDP-43 stress granular cells were treated with 70% ice-cold. measurements were performed on a SPECTRAmax-340 plate reader (Molecular Devices Inc. control. Bertin Precellys Evolution Touch Homogenizer. Ehrlich assay for quantification of 5-ALA A 200 μL 5-ALA sample was mixed with 200 μL sodium acetate (pH 4. Subsequent replacements require only the lamp (N-34-LB) be replaced. Rapid, accurate DNA, RNA, and protein quantitation in a one-touch, full-spectrum micro-volume absorbance reader. 003 OD Stray light ≤ 0. Reagena Poc Puumala® was used for detection of IgM antibody against PUUV in NE patient sera at the Department of Clinical Virology,. Centrifuge – Eppendorf 5417C with rotor FA 45–30–11. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. . The reaction mixture was incubated for 5 min. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. 2 were considered negative for IgM antibody. 4; 100 m M NaCl, 0. Manager: Yinghua Chen, PhD Contact Email: yinghua. Details. 5 mL sample or standard solution was well mixed with 0. The supernatants were mixed with an LDH assay reagent, which was purchased from Takara Bio Inc. (1988) and recorded at 412 nm. SpectraMax Multi-Mode Microplate Readers. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. 2. They are for reference only. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Apoptosis was also measured by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) assay using the TACS Blue label kit (4811-30-K, R&D Systems). 5. MCE has not independently confirmed the accuracy of these methods. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideHigh-throughput assays for promiscuous inhibitors Brian Y Feng1,3, Anang Shelat1,3, Thompson N Doman2,RKipGuy1 & Brian K Shoichet1 High-throughput screening (HTS) searches large libraries of chemical compounds. This is equivalent to having 511 built-in filters,Results: A total of 64% of the subjects reported improved quality of sleep during treatment with naloxone at a dose of 40 ng per 24 hours as compared with 9% with sham treatment (P=0. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. Absorbance values were read at 570 nm on an automated SpectraMAX 340 (Molecular Devices, Sunnyvale, CA, USA). control. , Fremont, CA, USA). Microplate Reader Shelf. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Setup recommendations for SpectraMax. D. Subsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. 25–0.